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KMID : 0613820110210030368
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Journal of Life Science
2011 Volume.21 No. 3 p.368 ~ p.374
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Response of Monodehydroascorbate Reductase in Lettuce Leaves Subjected to Low Temperature Stress
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Kang Sang-Jae
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Abstract
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The relationship between MDHA reductase activity and ascorbate, dehydroascorbate, and hydrogen peroxide content was evaluated, and this experiment was conducted to determine the change of MDHA reductase activity and the level of steady-state mRNA abundance of MDHA reductase in lettuce leaves subjected to low temperature stress. MDHA reductase activity of chloroplastic and cytosolic fraction in lettuce leaves subjected to 4℃ for 24 hr increased, followed by a steady decrease during the duration of recovery to 20℃ for 48 hr. The content of ascorbate slowly increased during low temperature treatment, followed by a rapid increase during the duration of recovery to 20℃ for 48 hr, while dehydroascorbate content rapidly decreased. The relationship between MDHA reductase activity of chloroplastic and cytosolic fraction in lettuce leaves subjected to 4℃ and ascorbate content correlated positively (R²=0.9240, 0.9108, respectively), but MDHA reductase activity of chloroplastic and cytosolic fraction and dehydroascorbate were reversely correlated (R²=0.8638, 0.8980, respectively). Hydrogen peroxide content and MDHA reductase activity of chloroplastic and cytosolic fraction in lettuce leaves subjected to 4℃ correlated positively (R²=0.9443, 0.9647, respectively). Northern blot analysis showed that the level of mRNA transcript of MDHA reductase was similar to total activity of MDHA reductase, and also that the level of mRNA of MDHA reductase after recovery to 20℃ for 24 hr decreased.
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KEYWORD
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Ascorbate, dehydroascorbate, hydrogen peroxide, MDHA reductase
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